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- Title
Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network
- Authors
Vladimirou, Elina; Li, Michael; Aldridge, Cassie P.; Frigerio, Lorenzo; Kirkilionis, Markus; Robinson, Colin
- Abstract
Abstract: The thylakoid membrane forms stacked thylakoids interconnected by ‘stromal’ lamellae. Little is known about the mobility of proteins within this system. We studied a stromal lamellae protein, Hcf106, by targeting an Hcf106-GFP fusion protein to the thylakoids and photobleaching. We find that even small regions fail to recover Hcf106-GFP fluorescence over periods of up to 3min after photobleaching. The protein is thus either immobile within the thylakoid membrane, or its diffusion is tightly restricted within distinct regions. Autofluorescence from the photosystem II light-harvesting complex in the granal stacks likewise fails to recover. Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming ‘microdomains’ that are sharply separated.
- Subjects
MEMBRANE proteins; THYLAKOIDS; GREEN fluorescent protein; PHOTOSYNTHETIC reaction centers; BLEACHING (Chemistry); PHOTOCHEMISTRY
- Publication
FEBS Letters, 2009, Vol 583, Issue 22, p3690
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.10.057