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- Title
Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching
- Authors
Miloslavina, Yuliya; Wehner, Antje; Lambrev, Petar H.; Wientjes, Emilie; Reus, Michael; Garab, Győző; Croce, Roberta; Holzwarth, Alfred R.
- Abstract
Abstract: Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll–chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl–Chl charge transfer state which plays a crucial part in the quenching.
- Subjects
FLUORESCENCE; SPECTRUM analysis; OLIGOMERS; CHLOROPHYLL; ARABIDOPSIS; CHARGE transfer in biology
- Publication
FEBS Letters, 2008, Vol 582, Issue 25/26, p3625
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.09.044