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- Title
Mechano-chemical effects of Ca<sup>2+</sup> in cross-linked troponin-C films
- Authors
Bukatina, Anna E.; Morozov, Victor N.; Gusev, Nikolai B.; Sieck, Gary C.
- Abstract
Changes in troponin C (TnC) conformation upon Ca2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca2+ binding to the Ca2+/Mg2+ sites of TnC.
- Subjects
CALCIUM ions; THIN films
- Publication
FEBS Letters, 2002, Vol 524, Issue 1-3, p107
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03013-2