We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Determination of Fucose Concentration in a Lectin-Based Displacement Microfluidic Assay.
- Authors
Erlandsson, Per G.; Åström, Eva; Påhlsson, Peter; Robinson, Nathaniel D.
- Abstract
We compare three different methods to quantify the monosaccharide fucose in solutions using the displacement of a large glycoprotein, lactoferrin. Two microfluidic analysis methods, namely fluorescence detection of (labeled) lactoferrin as it is displaced by unlabeled fucose and the displacement of (unlabeled) lactoferrin in SPR, provide fast responses and continuous data during the experiment, theoretically providing significant information regarding the interaction kinetics between the saccharide groups and binding sites. For comparison, we also performed a static displacement ELISA. The stationary binding site in all cases was immobilized S2-AAL, a monovalent polypeptide based on Aleuria aurantia lectin. Although all three assays showed a similar dynamic range, the microfluidic assays with fluorescent or SPR detection show an advantage in short analysis times. Furthermore, the microfluidic displacement assays provide a possibility to develop a one-step analytical platform.
- Subjects
LACTOFERRIN; FUCOSE; LECTINS; MICROFLUIDIC analytical techniques; BINDING sites; SACCHARIDES; SURFACE plasmon resonance
- Publication
Applied Biochemistry & Biotechnology, 2019, Vol 188, Issue 3, p868
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-018-02944-5