We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Synthetic Membraneless Droplets for Synaptic‐Like Clustering of Lipid Vesicles.
- Authors
Li, Qingchuan; Song, Qingchun; Guo, Wei; Cao, Yang; Cui, Xinyu; Chen, Dairong; Shum, Ho Cheung
- Abstract
In eukaryotic cells, the membraneless organelles (MLOs) formed via liquid‐liquid phase separation (LLPS) are found to interact intimately with membranous organelles (MOs). One major mode is the clustering of MOs by MLOs, such as the formation of clusters of synaptic vesicles at nerve terminals mediated by the synapsin‐rich MLOs. Aqueous droplets, including complex coacervates and aqueous two‐phase systems, have been plausible MLO‐mimics to emulate or elucidate biological processes. However, neither of them can cluster lipid vesicles (LVs) like MLOs. In this work, we develop a synthetic droplet assembled from a combination of two different interactions underlying the formation of these two droplets, namely, associative and segregative interactions, which we call segregative‐associative (SA) droplets. The SA droplets cluster and disperse LVs recapitulating the key functional features of synapsin condensates, which can be attributed to the weak electrostatic interaction environment provided by SA droplets. This work suggests LLPS with combined segregative and associative interactions as a possible route for synaptic clustering of lipid vesicles and highlights SA droplets as plausible MLO‐mimics and models for studying and mimicking related cellular dynamics.
- Subjects
SYNAPTIC vesicles; NERVE endings; PHASE separation; LIPIDS; EUKARYOTIC cells; DROPLETS; BOSE-Einstein condensation
- Publication
Angewandte Chemie, 2023, Vol 135, Issue 45, p1
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202313096