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- Title
In vitro cytotoxic effect of Trastuzumab in combination with Pertuzumab in breast cancer cells is improved by interleukin-2 activated NK cells.
- Authors
Asgari, Amir; Sharifzadeh, Sedigheh; Ghaderi, Abbas; Hosseini, Ahmad; Ramezani, Amin
- Abstract
Targeting erb-b2 receptor tyrosine kinase 2 (ERBB2) using the combination of Trastuzumab and Pertuzumab has demonstrated promising results in breast cancer therapy. It has further been revealed that interleukin-2 (IL-2) can activate Natural Killer cells (NK cells) and elevate their cytotoxic potency against tumor cells. In this study, we explored the cytotoxic effect of recombinant human IL-2 in combination with Trastuzumab and Pertuzumab on the ERBB2 positive (SK-BR-3) and negative (MDA-MB-231) breast cancer cell lines. The cytotoxicity level of IL-2 activated NK cells (approximately 75%) were significantly higher than untreated cells (approximately 55%) in the presence of Trastuzumab and Pertuzumab against SK-BR-3 cells, while no difference was observed in the case of MDA-MB-231 cells (about 15%).
- Subjects
CANCER cells; TRASTUZUMAB; BREAST cancer; INTERLEUKIN-2; ANTIBODY-dependent cell cytotoxicity; KILLER cells; PROTEIN-tyrosine kinases
- Publication
Molecular Biology Reports, 2019, Vol 46, Issue 6, p6205
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-019-05059-0