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- Title
Treatment of osteoporosis with TheraCyte-encapsulated parathyroid cells: a study in a rat model.
- Authors
Chou, F.-F; Huang, S.-C.; Chen, S.-S.; Wang, P.-W.; Huang, P.-H.; Lu, K.-Y.
- Abstract
Introduction: The purpose of this study was to evaluate parathyroid function at monthly intervals following the implantation of TheraCyte-encapsulated live human parathyroid cells into ovariectomized rats and to determine the effect on bone mineral density (BMD) 4 months after ovariectomy ( 3 months after implantation). Methods: Parathyroid tissues were obtained from patients undergoing surgery for secondary hyperparathyroidism. In total, 21 Sprague-Dawley rats divided randomly into three groups were subjected to one of three treatments: (1) implanted with TheraCyte A-encapsulated 4 x 106 live parathyroid cells; (2) implanted with TheraCyte B-encapsulated 4 x 105 live parathyroid cells; (3) a sham operation; the control group. Rats were ovariectomized 1 month prior to the implantation of the TheraCyte. Blood was drawn at the time of implantation and at monthly intervals thereafter for 3 months to check the levels of calcium, phosphorus and intact parathyroid hormone (iPTH). The BMD of the lumbar spine (L1-L5) and of the left femoral bone was measured with dual-energy-X-ray absorptiometry (DEXA) 1 month after ovariectomy and 3 months after implantation of the TheraCyte (4 months after ovariectomy). Results: We found that the viability ratio of cryopreserved tissues was between 55 and 79% after thawing. In the control group, the BMD of the lumbar spine (L1-L5) had not decreased significantly (p = 0.237) nor had the BMD of the left femoral bone increased significantly (p = 0.063) 3 months after implantation. In the TheraCyte A group, the BMD of both the lumbar spine (p = 0.018) and left femoral bone (p = 0.018) had increased significantly 3 months after implantation. In the TheraCyte B group, the BMD of both the lumbar spine (p = 0.017) and the left femoral bone (p = 0.025) had also increased significantly 3 months after implantation. Serum iPTH levels were higher in the TheraCyte A group than in the TheraCyte B group (p =0.006), and higher in the TheraCyte B group than in the control group (p = 0.040). Serum calcium levels were not significantly higher in the TheraCyte group A than in the TheraCyte B group or in the control group. Serum phosphorus levels were not significantly different between the TheraCyte A and TheraCyte B groups. Conclusions: Implantation of TheraCyte A-encapsulated 4 x 105 live parathyroid cells and TheraCyte B-encapsulated 4 x 106 cells can increase the BMD of ovariectomized rats within 3 months of implantation. Neither cause high serum calcium and low phosphorus concentrations.
- Subjects
PARATHYROID gland diseases; OSTEOPOROSIS; BONE diseases; THERAPEUTICS; RATS
- Publication
Osteoporosis International, 2006, Vol 17, Issue 6, p936
- ISSN
0937-941X
- Publication type
Article
- DOI
10.1007/s00198-006-0080-7