We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genetic and genomic diversity of NheABC locus from Bacillus strains.
- Authors
Cai, Yan; Huang, Tingxuan; Xu, Yuekang; Zhou, Guoping; Zou, Ping; Zeng, Guifeng; Liu, Xiaojin
- Abstract
Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato ( B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurrence of NHE among them, and other members of Bacillus is unclear. Consequently, we aimed to determine the distribution and evolution of NHE within Bacillus by confirming the presence of the nheA, B and C sequences and variation within them using published data, and to analyze the genomic and genetic diversity. The phylogenetic tree of NHE proteins (NheA, NheB and NheC) from 81 different B. cereus s.l. strains was constructed. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis. Analysis of the genomic diversity of the nheA, B and C loci revealed that their upstream regions were more conserved than the downstream sequences. Multilocus sequence typing schemes (MLST) based on seven concatenated housekeeping genes and nheA, B and C genes of the 75 strains were developed. The neighbor joining phylogenetic tree based on seven housekeeping genes together with nheA, B and C genes was similiar to published Bacillus phylogenetic trees. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis.The results indicate that nheA, B and C genes do not affect the diversity of housekeeping genes, and this specific NHE protein does not participate in the diarrheic syndrome.
- Subjects
BACILLUS (Bacteria); ENTEROTOXINS; MICROBIAL diversity; MICROBIAL virulence; BACTERIA phylogeny
- Publication
Archives of Microbiology, 2017, Vol 199, Issue 5, p775
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-017-1350-9