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- Title
Application of Innovative 3D Pathological Tactic for Diagnosis of Organizing Pneumonia.
- Authors
YI HSIA; YU HSIN TSAI; PIN-KUEI FU; CHIH JUNG CHEN
- Abstract
Background/Aim: The pathological diagnosis of organizing pneumonia (OP) relies on conventional traditional histopathological analysis, which involves examining stained thin slices of tissue. However, this method often results in suboptimal diagnostic objectivity due to low tissue sampling rates. This study aimed to assess the efficacy of tissue-clearing and infiltration-enhanced 3D spatial imaging techniques for elucidating the tissue architecture of OP. Materials and Methods: H&E staining, 3D imaging technology, and AIassisted analysis were employed to facilitate the construction of a multidimensional tissue architecture using six OP patient specimens procured from Taichung Veterans General Hospital, enabling a comprehensive morphological assessment. Results: Specimens underwent H&E staining and exhibited Masson bodies and varying degrees of interstitial fibrosis. Furthermore, we conducted a comprehensive study of 3D images of the pulmonary histology reconstructed through an in-depth pathology analysis, and uncovered heterogenous distributions of fibrosis and Masson bodies across different depths of the OP specimens. Conclusion: Integrating 3D imaging for OP with AI-assisted analysis permits a substantially enhanced visualization and delineation of complex histological pulmonary disorders such as OP. The synergistic application of conventional histopathology with novel 3D imaging elucidated the sophisticated spatial configuration of OP, revealing the presence of Masson bodies and interstitial fibrosis. This methodology transcends conventional pathology constraints and paves the way for advanced algorithmic approaches to enhance precision in the detection, classification, and clinical management of lung pathologies.
- Subjects
ORGANIZING pneumonia; PNEUMONIA treatment; ARTIFICIAL intelligence in medicine; THREE-dimensional imaging; VISUALIZATION
- Publication
In Vivo, 2024, Vol 38, Issue 4, p1993
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.13656