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- Title
Outbreak of porcine epidemic diarrhoea virus (PEDV) in Abruzzi region, central‐Italy.
- Authors
Pizzurro, Federica; Cito, Francesca; Zaccaria, Guendalina; Spedicato, Massimo; Cerella, Angelo; Orsini, Massimiliano; Forzan, Mario; D'Alterio, Nicola; Lorusso, Alessio; Marcacci, Maurilia
- Abstract
Abstract: Here we report and characterize a porcine epidemic diarrhea (PED) outbreak which occurred in a swine fattening farm in the province of Teramo, Abruzzi region (central Italy), in January 2016. PED virus (PEDV) identification was determined by real‐time RT‐PCR performed on RNAs purified from fecal samples collected from two symptomatic pigs. Whole genome sequence (PEDV 1842/2016) was also obtained by next generation sequencing straight from RNA purified from one fecal sample. Genome comparison with extant global PEDV strains revealed a high nucleotide identity with recently reported European and American S‐INDEL PEDVs. Efficient sequencing, share of genomic data combined with the implementation of epidemiological tools would be the ideal approach for study and analysis of transboundary infectious diseases as PED.
- Subjects
ABRUZZO (Italy); PORCINE epidemic diarrhea virus; FECAL analysis; NUCLEOTIDE sequencing; POLYMERASE chain reaction
- Publication
Veterinary Medicine & Science, 2018, Vol 4, Issue 2, p73
- ISSN
2053-1095
- Publication type
Article
- DOI
10.1002/vms3.88