We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Low-Temperature Plasma Suppresses Proliferation and Induces Apoptosis in Lung Cancer Cells by Regulating the miR-203a/BIRC5 Axis.
- Authors
Yang, Yang; Li, Dan; Li, Yulong; Jiang, Qiuyu; Sun, Ruifang; Liu, Jinren; Wu, Fei; Miao, Jiyu; Ni, Lei; Shi, Xingmin; Huang, Chen
- Abstract
Aim: Low-temperature plasma (LTP) has potential applications in cancer therapy. Herein, we explored the molecular mechanisms of proliferation inhibition induced by LTP. Methods: LTP was generated by a helium atmospheric-pressure plasma jet and used to treat A549 and H1299 cells. CCK-8 and cell apoptosis assays were performed to evaluate the effects of LTP treatment on A549 and H1299 cells. The qRT-PCR was performed to measure the expression of miR-203a after treating with LTP. CCK-8, colony formation, cell apoptosis assays, and Western blotting were performed to analyse the function of miR-203a in the development of lung cancer. Dual-luciferase assay and Western blotting were used to probe the relationship between miR-203a and BIRC5, and gene silencing using si-BIRC5 was carried out to explore the effect of knocking down BIRC5 on lung cancer cells. Results: We found that LTP significantly suppressed proliferation and promoted apoptosis in A549 and H1299 cells. The miR-203a expression was increased after cells were treated with LTP. The miR-203a expression was downregulated among lung cancer tissue samples, and overexpression of miR-203a suppressed cell growth and induced apoptosis in lung cancer cells. We showed that miR-203a targeted BIRC5. Moreover, silencing of BIRC5 caused proliferation inhibition and induced apoptosis in lung cancer cells. Conclusion: Our study revealed that LTP inhibited proliferation and induced apoptosis in A549 and H1299 cells through the miR-203a/BIRC5 axis. These findings showed that LTP could potentially be used to treat lung cancer.
- Subjects
CANCER cells; LUNG cancer; APOPTOSIS inhibition; PLASMA jets; APOPTOSIS
- Publication
OncoTargets & Therapy, 2020, Vol 13, p5145
- ISSN
1178-6930
- Publication type
Article
- DOI
10.2147/OTT.S244853