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- Title
Newly developed SNP markers related to genes for leaf morphological traits and disease resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis).
- Authors
Ahn, Yul-Kyun; Cho, Young-Il; Tripathi, Swati; Kim, Do-Sun; Kim, Jeong-Ho; Lee, Hye-Eun
- Abstract
Based on the sequences of Brassica rapa ssp. pekinensis, we carried out a single-nucleotide polymorphism (SNP) study for genes controlling disease resistance (clubroot, turnip mosaic virus and soft rot) and leaf traits in B. rapa leafy genotypes ‘VC40’ and ‘SR5’. In total, 7645 SNP markers were obtained based on the annotation of a B. rapa database for disease resistance. Among these 7645 SNP markers, 141 were related to 125 genes linked to leaf traits. From these 141 SNP markers, 63 were screened and High Resolution Melt (HRM) primers were designed for genomic studies. A total of 20 polymorphic SNP primers were finally obtained. Eventually, these markers will be further used for the detection of quantitative trait loci and mapping studies.
- Subjects
SINGLE nucleotide polymorphisms; CABBAGE disease &; pest resistance; CHINESE cabbage; CABBAGE varieties; PLANT species; TURNIP mosaic virus
- Publication
Plant Genetic Resources: Characterisation & Utilisation, 2014, Vol 12, Issue S1, pS62
- ISSN
1479-2621
- Publication type
Article
- DOI
10.1017/S1479262114000288