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- Title
Transplantation of Pancreatic Islets Contained in Minimal Volume Microcapsules in Diabetic High Mammalians.
- Authors
CALAFIORE, RICCARDO; BASTA, GIUSEPPE; LUCA, GIOVANNI; BOSELLI, CARLO; BUFALARI, ANDREA; BUFALARI, ANTONELLO; CASSARANI, MARIA PAOLA; GIUSTOZZI, GIAN MARIO; BRUNETTI, PAOLO
- Abstract
ABSTRACT: To minimize technical problems relating to excessive size (600-800μ in diameter) of standard alginate microcapsules (CSM) for pancreatic islet graft immunoisolation, we have developed two novel minimal volume, chemically identical, capsule prototypes (MVC): 1) coherent microcapsules (CM), and 2) medium-size microcapsules (300-400μ, MSM). CM, which envelop each individual islet within a thin alginate hydrogel cast, are prepared by emulsification, whereas MSM are made by atomizing the islet-alginate suspension through a special microdroplet generator. Upon graft into diabetic rodents, CM have shown to immunoprotect both allo- and xenogeneic nondiscordant islets, and restored normoglycemia. In higher mammals, at sub-therapeutic doses, CM fully immunoprotected islet allografts (pig→pig), but only temporarily xenografts (dog→pig). We then used MSM to immunoisolate canine islet allografts in the peritoneal cavity of dogs with spontaneous insulin-dependent diabetes. Of three grafted dogs, two showed full remission of hyperglycemia with insulin withdrawal. MSM could represent an intermediate solution between CSM and CM for peritoneal immunoisolated islet transplants.
- Publication
Annals of the New York Academy of Sciences, 1999, Vol 875, Issue 1, p219
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.1999.tb08506.x