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- Title
Identification of cachexia-inducible Hop stunt viroid variants in citrus orchards in Japan using biological indexing and improved reverse transcription polymerase chain reaction.
- Authors
Ito, Takao; Furuta, Takane; Ito, Tsutae; Isaka, Masahiro; Ide, Yoichi; Kaneyoshi, Junko
- Abstract
The distribution of Hop stunt viroid (HSVd) variants related to cachexia disease in Japan was investigated using reverse transcription polymerase chain reaction (RT-PCR) to specifically detect the five nucleotide differences in the “cachexia motif.” With RT-PCR, HSVd variants having the cachexia motif were detected in samples from 10 of 43 citrus trees in experimental orchards and from only 3 of 530 in commercial orchards. Although the trees were symptomless cachexia carriers, biological indexing using “Parson's Special” mandarin ( Citrus reticulata Blanco) verified the presence of the cachexia agent. RT-PCR with the primer pair CCV-M1 and CCV-P1 will be useful in preventing the spread of cachexia HSVd variants.
- Subjects
JAPAN; CACHEXIA; VIROID diseases of plants; CITRUS fruits; POLYMERASE chain reaction; MANDARIN orange; ORCHARDS; GENE therapy
- Publication
Journal of General Plant Pathology, 2006, Vol 72, Issue 6, p378
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-006-0303-y