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- Title
Investigation of the Relationship Between IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) Gene Variations and Serum Copper and Zinc Levels in Patients Diagnosed with Chronic Renal Failure.
- Authors
Ay, Arzu; Alkanli, Nevra; Ustundag, Sedat
- Abstract
The aim of this study is to investigate the relationship between IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations and serum trace element levels in patients diagnosed with CRF. Genotype distributions of IL-18 (− 607 C/A, − 137 G/C) gene variations were determined by polymerase chain reaction (PCR) method. PCR-restriction fragment length polymorphism (RFLP) methods were used to determine the MMP-2 (− 1306 C/T) gene variation genotype distributions. Serum trace element levels were determined by atomic absorption spectrophotometer method. A significant difference was found between the CRF patient and healthy control groups in terms of genotype distributions of IL-18 (− 607 C/A) and MMP-2 (− 1306 C/T) gene variations (p < 0.05). The significant difference was found between the patient and control groups in terms of serum copper and zinc levels and copper/zinc ratio (p < 0.05). The significant difference was detected between patient and control groups in terms of copper and zinc levels and copper/zinc ratio according to IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations and genotype distributions (p < 0.05). In addition, significant difference was determined in terms of serum copper and zinc levels and copper/zinc ratio according to haplotypes of IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations between patient and control groups (p < 0.05). In conclusion, evaluation of IL-18 (− 607 C/A, − 137 G/C) and MMP-2 (− 1306 C/T) gene variations and serum trace element levels together is extremely important in terms of obtaining important biomarkers in CRF early diagnosis and progression.
- Subjects
CHRONIC kidney failure; ZINC; COPPER; POLYMERASE chain reaction; GENES
- Publication
Biological Trace Element Research, 2022, Vol 200, Issue 5, p2040
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-021-02828-6