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- Title
Dihydromyricetin Exhibits Antitumor Activity in Nasopharyngeal Cancer Cell Through Antagonizing Wnt/β-catenin Signaling.
- Authors
Ye, Ling; Yin, Gendi; Jiang, Miaohua; Tu, Bo; Li, Zhicheng; Wang, Yiming
- Abstract
Background: Cancer stem cells (CSCs) have been demonstrated to play a vital role in a diversity of biological processes in cancers. With the emergence of new evidence, the important function of CSCs in the formation of multidrug resistance of nasopharyngeal cancer has been demonstrated. Dysregulated Wnt/β-catenin signaling pathway is an important contributor to chemoresistance and maintenance of CSCs-like characteristics. This research aims to investigate comprehensively the function of dihydromyricetin (DMY), a natural flavonoid drug, on the cisplatin (cis) resistance and stem cell properties of nasopharyngeal cancer. Methods: In this study, the functional role of DMY in nasopharyngeal cancer progression was comprehensively investigated in vitro and in vivo, and then its relationship with CSCs-like phenotypes and multiple oncogenes was analyzed. Results: In parallel assays, the growth inhibitory action of cis was enhanced by the addition of DMY in cis-resistant nasopharyngeal cancer cell lines (Hone1/cis and CNE1/cis). Functional assays showed that DMY markedly diminished the stem cell properties of nasopharyngeal cells, such as colony and tumor-sphere formation. In vivo data showed that the growth of Hone1 CSCs formed tumor xenograft was inhibited significantly by the administration of DMY. Additionally, DMY could impair the Wnt/β-catenin signaling pathway and regulate the expression of downstream proteins in nasopharyngeal cancer cells. Conclusions: Our study clarified the anti-tumor activity of DMY through blocking the Wnt/β-catenin signaling pathway in nasopharyngeal cancer. Therefore, DMY could be a novel therapeutic agent for nasopharyngeal cancer treatment.
- Subjects
THERAPEUTIC use of antineoplastic agents; IN vitro studies; FLOW cytometry; FLAVONOIDS; IN vivo studies; XENOGRAFTS; ANALYSIS of variance; ONCOGENES; COLONY-forming units assay; WESTERN immunoblotting; WNT proteins; APOPTOSIS; CELLULAR signal transduction; CELL survival; CISPLATIN; STEM cells; FLUORESCENT antibody technique; DESCRIPTIVE statistics; CELL lines; DATA analysis software; NASOPHARYNX tumors; PHENOTYPES
- Publication
Integrative Cancer Therapies, 2021, Vol 20, p1
- ISSN
1534-7354
- Publication type
Article
- DOI
10.1177/1534735421991217