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- Title
LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma.
- Authors
Moura, Mirian R. L.; de Nucci, Gilberto; Rath, Susanne; Reyes, Felix G. R.
- Abstract
A liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric method (LC-APCI-MS-MS) for the determination of glybenclamide in human plasma is described. Glypizide, an analogue of glybenclamide, was used as internal standard. The analyte was extracted from plasma with diethyl ether/dichloromethane (70:30 v/v). The chromatography uses C18 and 0.01 mol L-1 acetic acid/acetonitrile (20:80 v/v) as stationary and mobile phase, respectively. Quantitation was preformed by using multiple reaction monitoring (MRM) of the precursor ion (m/z 494.2→368.8) and the related product ion (m/z 446.0→347.3) using the internal standard method. The analytical curve was linear in the range 1–300 ng mL-1, and for a 400-μL sample of human plasma, the limit of determination of the method was 1 ng mL-1. The coefficients of variation of the method for intra-assay (within-run precision) and inter-assay (between-run precision) were less than 10%. The method was shown to be suitable for pharmacokinetic studies.
- Subjects
CHROMATOGRAPHIC analysis; ANALYTICAL chemistry; CHROMATOGRAMS; ACETIC acid; FATTY acids; CHLOROACETIC acids
- Publication
Analytical & Bioanalytical Chemistry, 2004, Vol 378, Issue 2, p499
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-2248-8