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- Title
Untitled.
- Authors
Lei, Chenghong; Wollenberger, Ulla; Bistolas, Nikitas; Guiseppi-Elie, Anthony; Scheller, Frieder
- Abstract
Nanostructured sodium montmorillonite was prepared via a colloidal chemical approach and deposited onto glassy carbon electrodes (GCE). Subsequently, hemoglobin was spontaneously adsorbed onto the clay membrane-modified electrode. The colloidal clay nanoparticles and the adsorbed protein were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrochemical impedance behavior of the system was studied using a microlithographically fabricated interdigitated microsensor electrode (IME). The interaction of the clay nanoparticles with hemoglobin was investigated by UV-VIS spectroscopy and electrochemical methods. The heme protein adsorbed in this way displayed a well-defined electrode process and the electron transfer was confirmed to originate from its heme site. Furthermore, nitric oxide affects the hemoglobin electrochemistry.
- Publication
Analytical & Bioanalytical Chemistry, 2002, Vol 372, Issue 2, p235
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-001-1200-z