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- Title
Galactose Oxidase/Prussian Blue Based Biosensors.
- Authors
Charmantray, Franck; Touisni, Nadia; Hecquet, Laurence; Noguer, Thierry; Mousty, Christine
- Abstract
This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-( O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag-AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx-TK biosensor.
- Subjects
GALACTOSE oxidase; TRANSKETOLASE; PRUSSIAN blue; LAYERED double hydroxides; ELECTROCHEMICAL analysis
- Publication
Electroanalysis, 2015, Vol 27, Issue 6, p1341
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201400720