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- Title
Dense gene physical maps of the non-model species Drosophila subobscura.
- Authors
Orengo, Dorcas; Puerma, Eva; Papaceit, Montserrat; Segarra, Carmen; Aguadé, Montserrat
- Abstract
The comparative analysis of genetic and physical maps as well as of whole genome sequences had revealed that in the Drosophila genus, most structural rearrangements occurred within chromosomal elements as a result of paracentric inversions. Genome sequence comparison would seem the best method to estimate rates of chromosomal evolution, but the high-quality reference genomes required for this endeavor are still scanty. Here, we have obtained dense physical maps for Muller elements A, C, and E of Drosophila subobscura, a species with an extensively studied rich and adaptive chromosomal polymorphism . These maps are based on 462 markers: 115, 236, and 111 markers for elements A, C, and E, respectively. The availability of these dense maps will facilitate genome assembly and will thus greatly contribute to obtaining a good reference genome, which is a required step for D. subobscura to attain the model species status. The comparative analysis of these physical maps and those obtained from the D. pseudoobscura and D. melanogaster genomes allowed us to infer the number of fixed inversions and chromosomal evolutionary rates for each pairwise comparison. For all three elements, rates inferred from the more closely related species were higher than those inferred from the more distantly related species, which together with results of relative-rate tests point to an acceleration in the D. subobscura lineage at least for elements A and E.
- Subjects
GENE mapping; DROSOPHILA subobscura; SPECIES diversity; NUCLEOTIDE sequencing; BIOMARKERS
- Publication
Chromosome Research, 2017, Vol 25, Issue 2, p145
- ISSN
0967-3849
- Publication type
Article
- DOI
10.1007/s10577-016-9549-1