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- Title
Molecular characterization of polyphosphate kinase (ppk) gene from Serratia marcescens.
- Authors
Seung-Jin Lee; Ok-Ryul Song; Young-Choon Lee; Yong-Lark Choi
- Abstract
To understand the mechanism of phosphate accumulation, a gene encoding polyphosphate kinase (PPK) was cloned from the genomic library of Serratia marcescens by Southern hybridization. From the nucleotide sequence of a 4 kb DNA fragment, an open reading frame of 2063 nucleotides was identified encoding a protein of 686 amino acids with molecular mass of 70 kDa. The potential CRP binding site and pho box sequence were found upstream of the putative promoter in the regulatory region. The expression of PPK resulted in the formation of inclusion bodies and the product was active at low temperature. The E. coli strain harboring plasmid pSPK5 with ppk gene increased enzyme activity of polyphosphate kinase, resulting in increased accumulation of polyphosphate in E. coli.
- Subjects
PHOSPHATES; SERRATIA marcescens; POLYPHOSPHATES; SOUTHERN blot; NUCLEOTIDE sequence; BIOTECHNOLOGY
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 3, p191
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1022386514487