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- Title
Production of 2-O-α-d-glucopyranosyl l-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp.
- Authors
Jun, Hong-Ki; Bae, Kyung-Mi; Kim, Sung-Koo
- Abstract
Transglycosylation to produce a 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) was studied using cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. A series of maltooligosaccharides substituted 2-O-derivatives of l-ascorbic acid (AA) were analyzed by HPLC. The maltooligosaccharides were hydrolyzed by glucoamylase to give AA-2G. CGTase also produced AA-2G using dextrin as a glycosyl donor and AA as an acceptor. CGTase utilized α-, β-, and γ-CDs, amylose, soluble starch and corn starch as glycosyl donors but not glucose.
- Subjects
GLYCOSYLTRANSFERASES; VITAMIN C; CYCLODEXTRINS; OLIGOSACCHARIDES; AMYLODEXTRINS; DEXTRINS
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 21, p1793
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1012413301061