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- Title
Iron sucrose and ferric carboxymaltose: no correlation between physicochemical stability and biological activity.
- Authors
Praschberger, Monika; Haider, Kathrin; Cornelius, Carolin; Schitegg, Markus; Sturm, Brigitte; Goldenberg, Hans; Scheiber-Mojdehkar, Barbara
- Abstract
Intravenous iron preparations, like iron sucrose (IS) and ferric carboxymaltose (FCM) differ in their physicochemical stability. Thus differences in storage and utilization can be expected and were investigated in a non-clinical study in liver parenchyma HepG2-cells and THP-1 macrophages as models for toxicological and pharmacological target cells. HepG2-cells incorporated significant amounts of IS, elevated the labile iron pool (LIP) and ferritin and stimulated iron release. HepG2-cells had lower basal cellular iron and ferritin content than THP-1 macrophages, which showed only marginal accumulation of IS and FCM. However, FCM increased the LIP up to twofold and significantly elevated ferritin within 24 h in HepG2-cells. IS and FCM were non-toxic for HepG2-cells and THP-1 macrophages were more sensitive to FCM compared to IS at all concentrations tested. In a cell-free environment redox-active iron was higher with IS than FCM. Biostability testing via assessment of direct transfer to serum transferrin did not reflect the chemical stability of the complexes (i.e., FCM > IS). Effect of vitamin C on mobilisation to transferrin was an increase with IS and interestingly a decrease with FCM. In conclusion, FCM has low bioavailability for liver parenchyma cells, therefore liver iron deposition is unlikely. Ascorbic acid reduces transferrin-chelatable iron from ferric carboxymaltose, thus effects on hepcidin expression should be investigated in clinical studies.
- Subjects
IRON; SUCROSE; MALTOSE; LIVER cells; TOXICOLOGY
- Publication
BioMetals, 2015, Vol 28, Issue 1, p35
- ISSN
0966-0844
- Publication type
Article
- DOI
10.1007/s10534-014-9801-0