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- Title
基于遗传多样性构建金针菇的核心种质群体及分 子身份证.
- Authors
高利慧; 鲍大鹏; 徐珍; 李燕; 陆欢; TAN Yee Shin; 尚晓冬; 陈洪雨; 王瑞娟; 吴莹莹
- Abstract
Flammulina filiformis is the high yield industry-cultivated edible mushroom in China.In order to improve the breeding efficiency of industry-cultivated F. filiformis germplasm, a total of 105 F. filiformis cultivated strains collected from China and oversea was used to analyse the genetic diversity using somatic incompatibility evaluation and SSR molecular markers. A total of 209 loci was amplified from 105 germplasms using 20 pairs of SSR primers. The genetic similarity coefficient of the all germplasms was ranged from 0.71 to 1.00, and the germplasms was divided into five groups with genetic distance of 0.76. All the 105 germplasms represented 67 sets of different genetic patterns and the genetic diversity of wild-type germplasm was more divergent than that of cultivated germplasm. The results of SSR cluster analysis and somatic incompatibility evaluation were correlated. In this study, the core collection of 44 germplasms (41.90% of the total germplasms) was constructed and the alleles were retained at 100%. The core collection revealed the genetic diversity and phenotypic variation of the 105 germplasms, and could provide a reference for parent selection in future breeding programme. The information of SSR fingerprinting, collection site, fruiting body colour and cultivation traits for each germplasm were constructed in form of QR code for easy identification and traceability purposes.
- Subjects
CHINA; GENETIC variation; FRUITING bodies (Fungi); TWO-dimensional bar codes; MICROSATELLITE repeats; EDIBLE mushrooms; GENETIC distance; CHLOROPLAST DNA
- Publication
Mycosystema, 2021, Vol 40, Issue 12, p3214
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.210317