We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Isolation and characterisation of crocosin, an antibacterial compound from crocodile ( Crocodylus siamensis) plasma.
- Authors
PREECHARRAM, Sutthidech; JEARRANAIPREPAME, Pornpimol; DADUANG, Sakda; TEMSIRIPONG, Yosapong; SOMDEE, Theerasak; FUKAMIZO, Tamo; SVASTI, Jisnuson; ARAKI, Tomohiro; THAMMASIRIRAK, Sompong
- Abstract
An antibacterial compound from crocodile blood was partially purified and functionally characterised. The freshwater crocodile ( Crocodylus siamensis) plasma with antibacterial activity was partially purified by using a centrifugal concentrator and reverse phase high powered liquid chromatography, and designated as crocosin. Crocosin exhibits antibacterial activity toward Salmonella typhi and Staphylococcus aureus. Crocosin is thermostable and resistant to pronase digestion. The structure of crocosin analyzed by mass spectrometry contains repeating units of 94 and 136 m/z. Scanning electron microscopy indicates that crocosin probably penetrates progressively into cytoplasm space, perturbing and damaging bacterial membranes. Crocosin may provide an early defense mechanism toward bacterial infection in freshwater.
- Subjects
CROCODYLUS; ANTIBACTERIAL agents; HIGH performance liquid chromatography; BLOOD plasma; BACTERIAL disease prevention; SALMONELLA typhi; STAPHYLOCOCCUS aureus
- Publication
Animal Science Journal, 2010, Vol 81, Issue 3, p393
- ISSN
1344-3941
- Publication type
Article
- DOI
10.1111/j.1740-0929.2010.00752.x