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- Title
Mitochondrial DNA-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination.
- Authors
Blomme, Jonas; Aken, Olivier Van; Leene, Jelle Van; Jégu, Teddy; Rycke, Riet De; Bruyne, Michiel De; Vercruysse, Jasmien; Nolf, Jonah; Daele, Twiggy Van; Milde, Liesbeth De; Vermeersch, Mattias; Francs-Small, Catherine Colas des; Jaeger, Geert De; Benhamed, Moussa; Millar, A. Harvey; Inzé, Dirk; Gonzalez, Nathalie
- Abstract
In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain- and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.
- Subjects
MITOCHONDRIAL proteins; MITOCHONDRIAL DNA; PLANT genomes; DNA repair; PLANT mitochondria; GENE expression
- Publication
Plant Cell, 2017, Vol 29, Issue 5, p1137
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1105/tpc.16.00899