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- Title
Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport in Saccharomyces cerevisiae.
- Authors
Chengqiang Wang; Yu Shen; Yanyan Zhang; Fan Suo; Jin Hou; Xiaoming Bao
- Abstract
The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h-1, a maximum specific L-arabinose consumption rate of 0.61 gh-1 g-1 dry cell weight, and a promising ethanol yield of 0.43 gg-1 from L-arabinose fermentation.
- Publication
BioMed Research International, 2013, Vol 2013, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2013/461204