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- Title
Growth of Endothelial Cells on Different Concentrations of Gly-Arg-Gly-Asp Photochemically Grafted in Polyethylene Glycol Modified Polyurethane.
- Authors
Lin, Yeong-Shang; Wang, Shoei-Shen; Chung, Tze-Wen; Wang, Yu-Hsung; Chiou, Sau-Hwa; Hsu, Jen-Ji; Chou, Nai-Kuan; Hsieh, Kou-Huang; Chu, Shu-Hsun
- Abstract
Abstract: To improve endothelial cell adhesion and growth on the surface of polyethylene glycol modified polyurethane (PU-PEG), cell adhesive peptide Gly-Arg-Gly-Asp (GRGD) was photochemically grafted to the surface. The surface grafted GRGD-N-Succinimidyl-6-[4′-azido-2′-nitrophenylamino]hexanoate (SANPAH) on a PU-PEG surface was performed by adsorption and subsequent ultraviolet irradiation. Fourier transform infrared spectra (FTIR) and electron spectroscopy for chemical analysis (ESCA) confirmed the GRGD grafted to form a PU-PEG-GRGD surface. The composition fraction of nitrogen calculated from ESCA analysis for the PU-PEG-GRGD surface was well correlated with the concentration of GRGD to be immobilized. Human umbilical vein endothelial cells (ECs) were well adhered and growing on the PU-PEG-GRGD surface. Moreover, the viability of ECs growing on PU-PEG-GRGD surfaces, analyzed by MTT test, was also well correlated with the GRGD concentrations immobilized on the surface. With photochemical techniques, we could manipulate different contents of GRGD to form multiple regions of PU-PEG-GRGD surface that could enhance the growth of ECs on the surface, and the enhancement efficiency was well correlated with GRGD contents.
- Subjects
POLYETHYLENE glycol; POLYURETHANES; ARTIFICIAL organs
- Publication
Artificial Organs, 2001, Vol 25, Issue 8, p617
- ISSN
0160-564X
- Publication type
Article
- DOI
10.1046/j.1525-1594.2001.025008617.x