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- Title
A reusable evanescent wave immunosensor for highly sensitive detection of bisphenolAin water samples.
- Authors
Zhou Xiao-hong; Liu Lan-hua; Xu Wei-qi; Song Bao-dong; Sheng Jian-wu; He Miao; Shi Han-chang
- Abstract
This paper proposed a compact and portable planar waveguide evanescent wave immunosensor (EWI) for highly sensitive detection of BPA. The incident light is coupled into the planar waveguide chip via a beveled angle through undergoing total internal reflection, where the evanescent wave field forms and excites the binding fluorophore-tagged antibodies on the chip surface. Typical calibration curves obtained for BPA has detection limits of 0.03 μg/L. Linear response for BPA ranged from 0.124 μg/L-9.60 μg/L with 50% inhibition concentration for BPA of 1.09±0.25 μg/L. The regeneration of the planar optical waveguide chip allows the performance of more than 300 assay cycles within an analysis time of about 20 min for each assay cycle. By application of effective pretreatment procedure, the recoveries of BPA in real water samples gave values from 88.3% ± 8.5% to 103.7% ± 3.5%, confirming its application potential in the measurement of BPA in reality.
- Subjects
BISPHENOL A; INTERNAL reflection spectroscopy; FLUOROPHORE synthesis; OPTICAL planar waveguides; GAS chromatography/Mass spectrometry (GC-MS)
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep04572