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- Title
MMP-2 mediates ethanol-induced invasion of mammary epithelial cells over-expressing ErbB2.
- Authors
Ke, Zunji; Lin, Hong; Fan, Zhiqin; Cai, Tian-Quan; Kaplan, Rebecca A.; Ma, Cuiling; Bower, Kimberly A.; Shi, Xianglin; Luo, Jia
- Abstract
Ethanol is a tumor promoter and may enhance the metastasis of breast cancer. We have previously demonstrated that over-expression of ErbB2 promoted ethanol-mediated invasion of mammary epithelial cells and breast cancer cells. However, the underlying cellular/molecular mechanisms remain unknown. By gelatin zymography, we showed that over-expression of ErbB2 increased the production of matrix metalloproteinase-2 (MMP-2) and MMP-9 in human mammary epithelial cells (HB2). Transient or stable transfection of ErbB2 cDNA to HB2 cells upregulated the transcripts and the activity of the MMP-2/-9 gene promoter; the upregulation of MMP-2/-9 expression was mediated by p38 mitogen-activated protein kinase (p38 MAPK) and phosphatidylinositol 3-kinase (PI3K). Although ethanol, at physiologically relevant concentrations (100-400 mg/dl), did not affect the production of MMP-2/-9, it activated MMP-2 in HB2 cells over-expressing ErbB2 (HB2
- Publication
International Journal of Cancer, 2006, Vol 119, Issue 1, p8
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.21769