We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Widening the landscape of transcriptional regulation of green algal photoprotection.
- Authors
Arend, Marius; Yuan, Yizhong; Ruiz-Sola, M. Águila; Omranian, Nooshin; Nikoloski, Zoran; Petroutsos, Dimitris
- Abstract
Availability of light and CO2, substrates of microalgae photosynthesis, is frequently far from optimal. Microalgae activate photoprotection under strong light, to prevent oxidative damage, and the CO2 Concentrating Mechanism (CCM) under low CO2, to raise intracellular CO2 levels. The two processes are interconnected; yet, the underlying transcriptional regulators remain largely unknown. Employing a large transcriptomic data compendium of Chlamydomonas reinhardtii's responses to different light and carbon supply, we reconstruct a consensus genome-scale gene regulatory network from complementary inference approaches and use it to elucidate transcriptional regulators of photoprotection. We show that the CCM regulator LCR1 also controls photoprotection, and that QER7, a Squamosa Binding Protein, suppresses photoprotection- and CCM-gene expression under the control of the blue light photoreceptor Phototropin. By demonstrating the existence of regulatory hubs that channel light- and CO2-mediated signals into a common response, our study provides an accessible resource to dissect gene expression regulation in this microalga. A consensus genome-scale gene regulatory network reconstruction allows dissection of the transcriptional regulation of microalgal responses to light and carbon availability and discovery of novel regulators of photoprotection and CO2 acquisition.
- Subjects
GENETIC transcription regulation; GENETIC regulation; CRYPTOCHROMES; GENE regulatory networks; CHLAMYDOMONAS reinhardtii; GERMPLASM
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-38183-4