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- Title
Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance.
- Authors
LaBarre, Jennifer L.; Hirschfeld, Emily; Soni, Tanu; Kachman, Maureen; Wigginton, Janis; Duren, William; Fleischman, Johanna Y.; Karnovsky, Alla; Burant, Charles F.; Lee, Joyce M.
- Abstract
As the incidence of obesity and type 2 diabetes (T2D) is occurring at a younger age, studying adolescent nutrient metabolism can provide insights on the development of T2D. Metabolic challenges, including an oral glucose tolerance test (OGTT) can assess the effects of perturbations in nutrient metabolism. Here, we present alterations in the global metabolome in response to an OGTT, classifying the influence of obesity and insulin resistance (IR) in adolescents that arrived at the clinic fasted and in a random-fed state. Participants were recruited as lean (n = 55, aged 8–17 years, BMI percentile 5–85%) and overweight and obese (OVOB, n = 228, aged 8–17 years, BMI percentile ≥ 85%). Untargeted metabolomics profiled 246 annotated metabolites in plasma at t0 and t60 min during the OGTT. Our results suggest that obesity and IR influence the switch from fatty acid (FA) to glucose oxidation in response to the OGTT. Obesity was associated with a blunted decline of acylcarnitines and fatty acid oxidation intermediates. In females, metabolites from the Fasted and Random-Fed OGTT were associated with HOMA-IR, including diacylglycerols, leucine/isoleucine, acylcarnitines, and phosphocholines. Our results indicate that at an early age, obesity and IR may influence the metabolome dynamics in response to a glucose challenge.
- Subjects
CHILDHOOD obesity; METABOLOMICS; PREPROCEDURAL fasting; SEX distribution; TYPE 2 diabetes; RISK assessment; COMPARATIVE studies; GLUCOSE tolerance tests; BODY mass index; INSULIN resistance; METABOLITES; FATTY acids; DISEASE risk factors; CHILDREN; ADOLESCENCE
- Publication
Nutrients, 2021, Vol 13, Issue 10, p3365
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu13103365