We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Bacillus subtilis IAB/BS03 as a potential biological control agent.
- Authors
Hinarejos, Estefanía; Castellano, Mayte; Rodrigo, Ismael; Bellés, José; Conejero, Vicente; López-Gresa, María; Lisón, Purificación
- Abstract
We describe the efficacy of Bacillus subtilis strain IAB/BS03 in reducing disease incidence of B. subtilis IAB/BS03 as a foliar treatment against Botrytis cinerea and Pseudomonas syringae on greenhouse-grown tomato ( Solanum lycopersicon) plants. We also tested the effect of foliar treatments on lettuce ( Lactuca sativa) against lettuce downy mildew caused by Bremia lactucae in multiple trials under different field conditions. All the assays indicated that B. subtilis IAB/BS03 reduced disease. To ascertain the mechanism of action, the induction of pathogenesis-related (PR) proteins, the accumulation of salicylic acid and the activation of peroxidase caused by foliar or root treatments with B. subtilis IAB/BS03 were studied in tomato. A salicylic acid-independent induction of the antifungal protein PR1 was observed after treatment with B. subtilis IAB/BS03, with the strongest induction due to root treatment compared with foliar application. A metabolic analysis of B. subtilis IAB/BS03 culture broth using Ultra Performance Liquid Chromatography coupled with ultraviolet and mass spectrometric detection determined surfactin and iturin A isomers. These compounds have been described as antifungal and antibiotic lipopeptides. The results indicated that B. subtilis IAB/BS03 could be effectively used as a biocontrol agent.
- Subjects
BACILLUS subtilis biotechnology; BIOLOGICAL control of tomato diseases &; pests; BOTRYTIS cinerea; ITURIN A; SURFACTIN; GREENHOUSE plants; FOLIAR application of agricultural chemicals
- Publication
European Journal of Plant Pathology, 2016, Vol 146, Issue 3, p597
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-016-0945-3