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- Title
Characterization of Agrobacterium species by capillary isoelectric focusing.
- Authors
Süle, Sándor; Horká, Marie; Matoušková, Hana; Kubesová, Anna; Salplachta, Jiří; Horký, Jaroslav
- Abstract
Tumorigenic and non-tumorigenic strains of Agrobacterium tumefaciens, A. rhizogenes, A.rubi, and A.vitis were examined using capillary isoelectric focusing, phenotypic determinative tests, PCR and fatty acid analysis. The isoelectric points (p I) of the 40 strains investigated clearly differentiated the strains according to their respective species. The different species were characterized with the following p I values: A. tumefaciens 2.2, A. rhizogenes 4.0, A. rubi 2.15, and A. vitis 2.6. This differentiation corresponded to the phenotypic, PCR and fatty acid characterizations. Strains with the similar chromosomal background but different plasmid content, e.g. A.vitis strain S4, and F2/5 gave the same p I values. Strains of Rhizobium species differed from Agrobacterium strains in their p I values. The advantage of capillary isoelectric focusing over the phenotypic determinative tests, PCR and fatty acid analysis is its speed (15 min), relative simplicity, and the very small amount of chemicals used. This rapid and simple method is a major improvement over the classical methods of separation of Agrobacterium species and should prove useful for rapid characterization of Agrobacterium-like colonies isolated from plant tumours for epidemiological and generic diversity studies.
- Publication
European Journal of Plant Pathology, 2012, Vol 132, Issue 1, p81
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-011-9850-y