We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nasopharyngeal Microbiota Profiles in Rural Venezuelan Children Are Associated With Respiratory and Gastrointestinal Infections.
- Authors
Verhagen, Lilly M; Rivera-Olivero, Ismar A; Clerc, Melanie; Chu, Mei Ling J N; Gastelaars, Jody van Engelsdorp; Kristensen, Maartje I; Berbers, Guy A M; Hermans, Peter W M; Jonge, Marien I de; Waard, Jacobus H de; Bogaert, Debby
- Abstract
Background Recent research suggests that the microbiota affects susceptibility to both respiratory tract infections (RTIs) and gastrointestinal infections (GIIs). In order to optimize global treatment options, it is important to characterize microbiota profiles across different niches and geographic/socioeconomic areas where RTI and GII prevalences are high. Methods We performed 16S sequencing of nasopharyngeal swabs from 209 Venezuelan Amerindian children aged 6 weeks–59 months who were participating in a 13-valent pneumococcal conjugate vaccine (PCV13) study. Using random forest models, differential abundance testing, and regression analysis, we determined whether specific bacteria were associated with RTIs or GIIs and variation in PCV13 response. Results Microbiota compositions differed between children with or without RTIs (P =.018) or GIIs (P =.001). Several species were associated with the absence of infections. Some of these health-associated bacteria are also observed in developed regions, such as Corynebacterium (log2(fold change [FC]) = 3.30 for RTIs and log2(FC) = 1.71 for GIIs), while others are not commonly observed in developed regions, such as Acinetobacter (log2(FC) = 2.82 and log2(FC) = 5.06, respectively). Klebsiella spp. presence was associated with both RTIs (log2(FC) = 5.48) and GIIs (log2(FC) = 7.20). Conclusions The nasopharyngeal microbiota of rural Venezuelan children included several bacteria that thrive in tropical humid climates. Interestingly, nasopharyngeal microbiota composition not only differed in children with an RTI but also in those with a GII, which suggests a reciprocal interplay between the 2 environments. Knowledge of region-specific microbiota patterns enables tailoring of preventive and therapeutic approaches.
- Subjects
VENEZUELA; NASOPHARYNX microbiology; STREPTOCOCCAL disease prevention; HUMAN microbiota; CORYNEBACTERIUM; GASTROINTESTINAL diseases; KLEBSIELLA; PNEUMOCOCCAL vaccines; REGRESSION analysis; RESPIRATORY infections; RURAL conditions; RANDOM forest algorithms; CHILDREN
- Publication
Clinical Infectious Diseases, 2021, Vol 72, Issue 2, p212
- ISSN
1058-4838
- Publication type
Article
- DOI
10.1093/cid/ciaa015