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- Title
Tryptophan within basic peptide sequences triggers glycosaminoglycan-dependent endocytosis.
- Authors
Bechara, Chérine; Pallerla, Manjula; Zaltsman, Yefim; Burlina, Fabienne; Alves, Isabel D.; Lequin, Olivier; Sagan, Sandrine
- Abstract
Deciphering the structural requirements and mechanisms for internalization of cell-penetrating peptides (CPPs) is required to improve their delivery efficiency. Herein, a unique role of tryptophan (Trp) residues in the interaction and structuring of cationic CPP sequences with glycosaminoglycans (GAGs) has been characterized, in relation with cell internalization. Using isothermal titration calorimetry, circular dichroism, NMR, mass spectrometry, and phase-contrast microscopy, we compared the interaction of 7 basic CPPs with 5 classes of GAGs. We found that the affinity of CPPs for GAGs increases linearly with the number of Trp residues, from 30 nM for a penetratin analog with 1 Trp residue to 1.5 nM for a penetratin analog with 6 . Trp residues for heparin (HI); peptides with Trp residues adopt a predominantly β-strand structure in complex with HI and form large, stable β-sheet aggregates with GAGs; and in the absence of any cytotoxicity effect, the quantity of peptide internalized into CHO cells increased 2 times with 1 Trp residue, 10 times with 2 Trp residues, and 20 times with 3 Trp residues, compared with +6 peptides with no Trp residues. Therefore, Trp residues represent molecular determinants in basic peptide sequences not only for direct membrane translocation but also for efficient endocytosis through GAGs.
- Subjects
TRYPTOPHAN; AMINO acid sequence; GLYCOSAMINOGLYCANS; ENDOCYTOSIS; ISOTHERMAL titration calorimetry; NUCLEAR magnetic resonance
- Publication
FASEB Journal, 2013, Vol 27, Issue 2, p738
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.12-216176