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- Title
Adverse effects of dietary glycotoxins on wound healing in genetically diabetic mice.
- Authors
Peppa, Melpomeni; Cai, Weijing; Zhu Li; Vlassara, Helen; Brem, Harold; Jian-Gang Zhang; Ehrlich, Patti; Croitoru, Anca; Thung, Swan; Ehrlich, Paul; Zhang, Jian-Gang; Li, Zhu
- Abstract
Advanced glycoxidation end products (AGEs) are implicated in delayed diabetic wound healing. To test the role of diet-derived AGE on the rate of wound healing, we placed female db/db (+/+) (n = 55, 12 weeks old) and age-matched control db/db (+/-) mice (n = 45) on two diets that differed only in AGE content (high [H-AGE] versus low [L-AGE] ratio, 5:1) for 3 months. Full-thickness skin wounds (1 cm) were examined histologically and for wound closure. Serum 24-h urine and skin samples were monitored for N(epsilon)-carboxymethyl-lysine and methylglyoxal derivatives by enzyme-linked immunosorbent assays. L-AGE-fed mice displayed more rapid wound closure at days 7 and 14 (P < 0.005) and were closed completely by day 21 compared with H-AGE nonhealed wounds. Serum AGE levels increased by 53% in H-AGE mice and decreased by 7.8% in L-AGE mice (P < 0.04) from baseline. L-AGE mice wounds exhibited lower skin AGE deposits, increased epithelialization, angiogenesis, inflammation, granulation tissue deposition, and enhanced collagen organization up to day 21, compared with H-AGE mice. Reepithelialization was the dominant mode of wound closure in H-AGE mice compared with wound contraction that prevailed in L-AGE mice. Thus, increased diet-derived AGE intake may be a significant retardant of wound closure in diabetic mice; dietary AGE restriction may improve impaired diabetic wound healing.
- Subjects
WOUND healing; PEOPLE with diabetes; LABORATORY mice
- Publication
Diabetes, 2003, Vol 52, Issue 11, p2805
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.52.11.2805