We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Ghrelin is present in pancreatic alpha-cells of humans and rats and stimulates insulin secretion.
- Authors
Date, Yukari; Nakazato, Masamitsu; Hashiguchi, Suzuko; Dezaki, Katsuya; Mondal, Muhtashan S; Hosoda, Hiroshi; Kojima, Masayasu; Kangawa, Kenji; Arima, Terukatsu; Matsuo, Hisayuki; Yada, Toshihiko; Matsukura, Shigeru
- Abstract
Ghrelin, a novel growth hormone-releasing peptide isolated from human and rat stomach, is a 28-amino acid peptide with a posttranslational acylation modification that is indispensable for stimulating growth hormone secretion by increasing intracellular Ca(2+) concentration. It also functions in the regulation of feeding behavior, energy metabolism, and gastric acid secretion and motility. Using two different antibodies against the NH(2)- and COOH-terminal regions of ghrelin, we studied its localization in human and rat pancreas by immunohistochemistry. Ghrelin-immunoreactive cells were identified at the periphery of pancreatic islets in both species. Ghrelin co-localized exclusively with glucagon in rat islets, indicating that it is produced in alpha-cells. We identified ghrelin and des-acyl ghrelin in the rat pancreas using reverse-phase high-performance liquid chromatography combined with two radioimmunoassays. We also detected mRNA encoding ghrelin and its receptor in the rat pancreatic islets. Ghrelin increased the cytosolic free Ca(2+) concentration in beta-cells and stimulated insulin secretion when it was added to isolated rat pancreatic islets. These findings indicate that ghrelin may regulate islet function in an endocrine and/or paracrine manner.
- Publication
Diabetes, 2002, Vol 51, Issue 1, p124
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.51.1.124