We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A rapid means of separating A14-125I-insulin from heterogeneously labeled insulin molecules for biologic studies.
- Authors
Stentz, Frankie B.; Wright, Reba K.; Kitabchi, Abbas E.; Stentz, F B; Wright, R K; Kitabchi, A E
- Abstract
We have used two methods for the preparation of a highly homogeneous insulin with high specific activity. After iodination with chloramine T, the labeled peptides were retained on a disposable Sep Pak cartridge and subsequently eluted. The eluted labeled insulins were further purified by either DEAE cellulose or high performance liquid chromatography (HPLC) to separate A14-125I- from A19-125I-insulin. Both methods of chromatography were effective, but HPLC offered the advantage of better resolution in less time and higher yields of A14-125I-insulin, which is suitable for biologic studies in various target tissues.
- Publication
Diabetes, 1982, Vol 31, Issue 12, p1128
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diacare.31.12.1128