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- Title
Quercetin modulates activities of Taiwan cobra phospholipase A via its effects on membrane structure and membrane-bound mode of phospholipase A.
- Authors
Chiou, Yi-Ling; Lin, Shinne-Ren; Hu, Wan-Ping; Chang, Long-Sen
- Abstract
The goal of the present study is to elucidate the mechanism of quercetin on modulating Naja naja atra phospholipase A (PLA) activities. Sphingomyelin inhibited PLA enzymatic activity and membrane-damaging activity against egg yolk phosphatidylcholine (EYPC), while cholesterol and quercetin abrogated the sphingomeyelin inhibitory effect. Quercetin incorporation led to a reduction in PLA enzymatic activity and membrane-damaging activity toward EYPC/sphingomyelin/cholesterol vesicles. Both cholesterol and quercetin increased detergent resistance and reduced membrane fluidity of EYPC/sphingomyelin vesicles. Quercetin reduced detergent insolubility but increased ordered lipid packing of EYPC/sphingomyelin/cholesterol vesicles. Acrylamide quenching studies and trinitrophenylation of Lys residues revealed that quercetin altered the membrane-bound mode of PLA differently upon absorption onto the membrane bilayers of different lipid compositions. However, 8-anilinonaphthalene sulphonate-binding assay revealed that quercetin marginally affected the interaction between active site of PLA with phospholipid vesicles. Collectively, our data indicate that membrane-inserted quercetin modulates PLA interfacial activity and membrane-damaging activity via its effects on membrane structure and membrane-bound mode of PLA.
- Subjects
QUERCETIN; PHOSPHOLIPASE A2 regulation; CHOLESTEROL; SPHINGOMYELIN; NAJA naja
- Publication
Journal of Biosciences, 2012, Vol 37, Issue 2, p277
- ISSN
0250-5991
- Publication type
Article
- DOI
10.1007/s12038-012-9198-2