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- Title
Effect of gemcitabine on the uptake of (18)F-fluorodeoxyglucose and (18)F-fluorothymidine in lung adenocarcinoma A549 cells and the animal tumor model.
- Authors
Bin Zhang; Sheng-Ming Deng; Ling-Chuan Guo; Jia-Jia Dong; Yan-Bo Zhu; Yuan Gao; Zhen-Xin Wang; Cho, William C.; Zhang, Bin; Deng, Sheng-Ming; Guo, Ling-Chuan; Dong, Jia-Jia; Zhu, Yan-Bo; Gao, Yuan; Wang, Zhen-Xin
- Abstract
<bold>Background: </bold>Gemcitabine is the first-line drug for nonsmall cell lung cancer, and 18F-fluorodeoxyglucose. (18F-FDG) and 18F-fluorothymidine. (18F-FLT) are positron emission tomography. (PET) imaging agents. The aim of this study was to explore the effect of gemcitabine on the uptake of 18F-FDG and 18F-FLT in A549 cells and the animal tumor model. <bold>Materials and Methods: </bold>The inhibitory effects of gemcitabine on cell growth were determined by tetrazolium blue method, and uptake rates of 18F-FDG and 18F-FLT were determined under the same conditions. The adenocarcinoma-bearing nude mice before and after gemcitabine treatments were performed microPET imaging with 18F-FDG and 18F-FLT. Hematoxylin and eosin staining and immunohistochemical analysis of tumor specimens were conducted. <bold>Results: </bold>After the administration of gemcitabine, positive correlations were observed between inhibition of 18F-FDG or 18F.FLT uptake and cell growth. (r = 0.957 or 0.981, P < 0.01). SUVmax values by 18F-FDG in the tumor, before and after administration of gemcitabine at the dose of 60 mmol/L, revealed an increase by. (35.83 ± 10.58) %. After administration of 120 mmol/L gemcitabine, the SUVmax values decreased by (12.37 ± 7.33) %. The SUVmax values by 18F-FLT at the dose of 60 mmol/L gemcitabine revealed a decrease by (56.47 ± 10.83) %. Pathological staining showed obvious vasodilation and invasion of lymphocytes and plasma cells at the dose of 60 mmol/L, and the expression of glucose transporter protein-1, Ki-67 and proliferating cell nuclear antigen in tumor cells were inhibited. <bold>Conclusion: </bold>18F-FLT imaging can assess the proliferation of tumor cells and 18F-FDG imaging can reflect the changes of the tumor microenvironment after administration of gemcitabine.
- Subjects
FLUORODEOXYGLUCOSE F18; NON-small-cell lung carcinoma; POSITRON emission tomography; TETRAZOLIUM; LABORATORY mice; IMMUNOHISTOCHEMISTRY; ADENOCARCINOMA; ANIMAL experimentation; ANIMALS; BIOLOGICAL models; CELL physiology; DEOXY sugars; LUNG cancer; LUNG tumors; MICE; RADIOPHARMACEUTICALS; DEOXYCYTIDINE
- Publication
Journal of Cancer Research & Therapeutics, 2016, Vol 12, Issue 1, p271
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.147713