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- Title
Synthesis of Folic Acid-Containing Imidazolium Copolymers for Potential Gene Delivery Applications.
- Authors
Allen, Michael H.; Day, Kelsea N.; Hemp, Sean T.; Long, Timothy E.
- Abstract
Folic acid conjugation onto poly(1-vinylimidazole) generates imidazolium copolymers for potential receptor-mediated nonviral gene delivery. Homopolymer quaternization with various tBoc-protected bromoalkylamines imparts a permanent charge for DNA complexation. Incorporation of primary amine groups provides a site for folic acid conjugation onto imidazolium copolymers. DNA binding, cytotoxicity, and in vitro transfection in HeLa cells reveal structure-property-transfection relationships for the imidazolium copolymers. Luciferase expression assays establish that primary amine conjugation onto imidazolium copolymers up to 30 mol% fails to improve transfection efficiency. In sharp contrast, incorporation of folic acid onto the copolymers improves transfection efficiency 250-fold.
- Publication
Macromolecular Chemistry & Physics, 2013, Vol 214, Issue 7, p797
- ISSN
1022-1352
- Publication type
Article
- DOI
10.1002/macp.201200613