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- Title
Design of Maleimide-Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups.
- Authors
Wright, Emma J.; Sosna, Maciej; Bloodworth, Sally; Kilburn, Jeremy D.; Bartlett, Philip N.
- Abstract
Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono- N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (≈7.5 pmol cm−2).
- Subjects
MONOMOLECULAR films; ACETAMIDE; HEXAMETHYLENEDIAMINE; CYTOCHROME c; ELECTRIC resistors; CYSTEINE; ELECTRODES
- Publication
Chemistry - A European Journal, 2014, Vol 20, Issue 19, p5550
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201400246