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- Title
The rice ( Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response.
- Authors
Fekih, Rym; Tamiru, Muluneh; Kanzaki, Hiroyuki; Abe, Akira; Yoshida, Kentaro; Kanzaki, Eiko; Saitoh, Hiromasa; Takagi, Hiroki; Natsume, Satoshi; Undan, Jerwin; Undan, Jesusa; Terauchi, Ryohei
- Abstract
Lesion mimic mutants (LMMs) provide a useful tool to study defense-related programmed cell death (PCD) in plants. Although a number of LMMs have been identified in multiple species, most of the candidate genes are yet to be isolated. Here, we report the identification and characterization of a novel rice ( Oryza sativa L.) lesion mimic resembling ( lmr) mutant, and cloning of the corresponding LMR gene. The LMR locus was initially delineated to 1.2 Mb region on chromosome 6, which was further narrowed down to 155-kb using insertions/deletions (INDELs) and cleavage amplified polymorphic sequence markers developed in this study. We sequenced the open reading frames predicted within the candidate genomic region, and identified a G-A base substitution causing a premature translation termination in a gene that encodes an ATPase associated with various cellular activities type (AAA-type) protein. RNA interference transgenic lines with reduced LMR transcripts exhibited the lesion mimic phenotype similar to that of lmr plants. Furthermore, expression of the wild-type LMR in the mutant background complemented the lesion phenotype, confirming that the mutation identified in LMR is responsible for the mutant phenotype. The pathogenesis-related (PR) genes PBZ1 and PR1 were induced in lmr, which also showed enhanced resistance to rice blast ( Magnaporthe oryzae) and bacterial blight ( Xanthomonas oryzae pv. oryzae), suggesting LMR is a negative regulator of cell death in rice. The identification of lmr and cloning of the corresponding LMR gene provide an additional resource for the study of PCD in plants.
- Subjects
RICE genetics; RNA interference; GENE expression in plants; ADENOSINE triphosphatase; PLANT mutation; DNA insertion elements
- Publication
Molecular Genetics & Genomics, 2015, Vol 290, Issue 2, p611
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-014-0944-z