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- Title
The combination of genetic variants of the FSHB and FSHR genes affects serum FSH in women of reproductive age.
- Authors
La Marca, A.; Papaleo, E.; Alviggi, C.; Ruvolo, G.; De Placido, G.; Candiani, M.; Cittadini, E.; De Michele, F.; Moriondo, V.; Catellani, V.; Volpe, A.; Simoni, M.
- Abstract
STUDY QUESTION What is the effect of FSHB-211G>T together with the FSHR 2039 A>G on serum FSH in women? SUMMARY ANSWER Serum FSH levels are affected by the combination of genetic polymorphisms in FSHR and FSHB. WHAT IS KNOWN ALREADY The relationship between SNPs of the FSHR gene and serum FSH has not been completely clarified. Genetic variants of the FSHB gene have been associated with variation in gene transcription and serum FSH levels in men. No data have been published on the effect of the FSHB-211G>T in women, alone or in combination with the FSHR 2039 A>G. STUDY DESIGN, SIZE, DURATION This study was a prospective study including 193 healthy women of reproductive age. PARTICIPANTS/MATERIALS, SETTING, METHODS Infertile and otherwise healthy eumenorrheic women (n = 193) with normal BMI and serum FSH levels were recruited for the study. In all women early follicular phase FSH and AMH were measured by commercial assays, and antral follicle count was measured by transvaginal ultrasound. Genomic DNA was purified from total peripheral blood and genotyping for the two SNPs was performed. MAIN RESULTS AND THE ROLE OF CHANCE No significant gradients of increasing or decreasing Day 3 FSH across the FSHR 2039 (AA/AG/GG) and FSHB-211 (GG/GT/TT) genotypes, respectively, were observed. When women were stratified according to the FSHR 2039, and FSHB-211 genotypes a statistically significant reduction of d3 FSH was shown in the group of women with the FSHB-211 GT + TT/FSHR2039 AA genotype compared with the FSHB-211 GG/FSHR2039 GG genotype, hence confirming a possible additive effect of the different SNPs in FSHR and FSHB on regulating serum FSH. LIMITATIONS, REASONS FOR CAUTION This finding requires an independent confirmation. However, it confirms the relationship between serum FSH and FSHB together with FSHR gene polymorphisms already reported in males. WIDER IMPLICATIONS OF THE FINDINGS The knowledge of the FSHB/FSHR genotype combination is fundamental for the proper interpretation of serum FSH levels in women of reproductive age. STUDY FUNDING/COMPETING INTERESTS Merck Serono supported the study in the form of a research grant for the laboratory session. None of the authors have any competing interest to declare.
- Subjects
HUMAN genetic variation; FOLLICLE-stimulating hormone; LUTEINIZING hormone releasing hormone; SERUM; MATERNAL age; GENETIC polymorphisms; GENETIC transcription; LONGITUDINAL method
- Publication
Human Reproduction, 2013, Vol 28, Issue 5, p1369
- ISSN
0268-1161
- Publication type
Article
- DOI
10.1093/humrep/det061