We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Применение гена argJ термофильной бактерии Geobacillus stearothermophilus для конструирования высокоактивного штамма-продуцента аргинина
- Authors
Колоян, А. О.; Аветисян, С. В.; Паронян, М. Г.; Овсепян, А. С.
- Abstract
Molecular cloning of the gene argJ of thermophilic bacterium Geobacillus stearothermophilus on Escherichia coli - Corynebacterim glutamicum shuttle expression vector pEC-XK99E in the cells of coryneform bacteria was carried out. To create a new arginine-producing strain, the constructed recombinant plasmid pARGJ was used. As a recipient, we used the previously obtained Brevibacterium flavum HK-19A strain, which synthesizes up to 25.0 g/L of L-arginine. The created new recombinant strain containing the heterologous gene argJ of the rmophilic bacterium provides higher yield (by 20%) of L-arginine compared to the recipient strain.
- Subjects
GEOBACILLUS stearothermophilus; ARGININE; ESCHERICHIA coli; BREVIBACTERIUM; BACTERIA; THERMOPHILIC bacteria; MOLECULAR cloning
- Publication
National Academy of Sciences of Armenia Reports / Doklady Nacionalnaâ Akademiâ Nauk Armenii, 2021, Vol 121, Issue 2, p133
- ISSN
1026-6496
- Publication type
Article