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- Title
Effects of Age, Postmortem Delay and Storage Time on Receptor-mediated Activation of G-proteins in Human Brain
- Authors
González-Maeso, Javier; Torre, Iratxe; Rodrıguez-Puertas, Rafael; Garcıa-Sevilla, Jesús A.; Guimón, José; Meana, J. Javier
- Abstract
The influence of age, postmortem delay and freezing storage period on receptor-mediated G-protein activity was quantified in cortical membranes from 34 healthy subjects. Concentration-response curves of the [35S]GTPγS binding stimulation by agonists for α2-adrenoceptors (UK14304), μ-opioid (DAMGO), 5-HT1A (8-OH-DPAT), GABAB (baclofen) and muscarinic (carbachol) receptors were analyzed. Immunoreactivities of Gα-protein subunits were also determined. Basal binding and UK14304, 8-OH-DPAT, and baclofen potency to stimulate [35S]GTPγS binding decreased with aging (1–88 years) without changes of efficacy. DAMGO-mediated stimulation increased both in potency and efficacy with aging. A negative correlation between age and immunoreactivity was observed for Gαi1/2-, but not for Gαi3-, Gαo-,and Gαs-proteins. Neither [35S]GTPγS binding nor Gα-proteins changed with the postmortem delay (8–92 h). Basal [35S]GTPγS binding decreased with the sample storage period (1–85 months). A careful match between cases and controls should be taken into account when designing signal transduction studies in human disorders, specially for variables such as age and storage period.
- Subjects
G proteins; COGNITION disorders
- Publication
Neuropsychopharmacology, 2002, Vol 26, Issue 4, p468
- ISSN
0893-133X
- Publication type
Article