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- Title
Differential proteome analysis of porcine skeletal muscles between Meishan and Large White.
- Authors
Xu, Y. J.; Jin, M. L.; Wang, L. J.; Zhang, A. D.; Zuo, B.; Xu, D. Q.; Ren, Z. Q.; Lei, M. G.; Mo, X. Y.; Li, F. E.; Zheng, R.; Deng, C. Y.; Xiong, Y. Z.
- Abstract
ABSTRACT: Western and indigenous Chinese pig breeds show obvious differences in muscle growth and meat quality; however, the underlying molecular mechanism remains unclear. In this study, proteome analysis of LM between purebred Meishan and Large White pigs was performed by 2-dimensional gel electrophoresis and mass spectrometry. A total of 25 protein spots were differentially expressed in the 2 breeds. The 14 identified proteins could be divided into 4 groups: energy metabolism, defense and stress, myofibrillar filaments, and other unclassified proteins. Quantitative real-time PCR was used to analyze the partly differentially cxpressed proteins in mRNA level, which revealed a positive correlation between the content of the proteins and their mRNA levels. We also analyzed the niRNA levels of myosin heavy chain isoforms using quantitative realtime PCR. The results indicated that ha and lix fibers were elevated in Meishan pigs, whereas the IIb fiber was more highly expressed in Large White pigs. TO the best of our knowledge, this was the first proteornicsbased investigation of total skeletal muscle protein in different pig breeds, and these results may provide valuable information for understanding the molecular mechanism responsible for breed-specific differences in growth performance and meat quality.
- Subjects
GEL electrophoresis; MASS spectrometry; SWINE; ENERGY metabolism; MESSENGER RNA; MYOSIN
- Publication
Journal of Animal Science, 2009, Vol 87, Issue 8, p2519
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2008-1708