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- Title
Propagation of centromeric chromatin requires exit from mitosis.
- Authors
Jansen, Lars E. T.; Foltz, Daniel R.; Cleveland, Don W.; Black, Ben E.
- Abstract
Centromeres direct chromosomal inheritance by nucleating assembly of the kinetochore, a large multiprotein complex required for microtubule attachment during mitosis. Centromere identity in humans is epigenetically determined, with no DNA sequence either necessary or sufficient. A prime candidate for the epigenetic mark is assembly into centromeric chromatin of centromere protein A (CENP-A), a histone H3 variant found only at functional centromeres. A new covalent fluorescent pulse-chase labeling approach using SNAP tagging has now been developed and is used to demonstrate that CENP-A bound to a mature centromere is quantitatively and equally partitioned to sister centromeres generated during S phase, thereby remaining stably associated through multiple cell divisions. Loading of nascent CENP-A on the megabase domains of replicated centromere DNA is shown to require passage through mitosis but not microtubule attachment. Very surprisingly, assembly and stabilization of new CENP-A-containing nucleosomes is restricted exclusively to the subsequent G1 phase, demonstrating direct coupling between progression through mitosis and assembly/maturation of the next generation of centromeres.
- Subjects
CHROMATIN; MITOSIS; CENTROMERE; NUCLEOTIDE sequence; CELL division
- Publication
Journal of Cell Biology, 2007, Vol 176, Issue 6, p795
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200701066