We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Low NETosis Induced in Anaplasma phagocytophilum -Infected Cells.
- Authors
Artigas-Jerónimo, Sara; González-García, Almudena; de la Fuente, José; Blanda, Valeria; Shekarkar Azgomi, Mojtaba; Villar, Margarita; Mohammadnezhad, Leila; Grippi, Francesca; Torina, Alessandra; Sireci, Guido
- Abstract
Anaplasma phagocytophilum are obligatory intracellular bacteria that preferentially replicate inside leukocytes by utilizing biological compounds and processes of these primary host defensive cells. In this study, bioinformatics analysis was conducted to further characterize A. phagocytophilum–host interactions using the neutrophil-like model of human Caucasian promyelocytic leukemia HL60 cells. We detected a hierarchy of molecules involved in A. phagocytophilum-HL60 interactions with overrepresentation in infected human cells of proteins involved in the reactive oxygen species (ROS) pathway and cell surface monocyte markers. As A. phagocytophilum phagocytosis by neutrophils is inhibited, the results suggested a possible explanation for our bioinformatics data: radical oxygen compounds could induce the killing of bacteria activating NETosis, a unique form of defense mechanism resulting in cell death that is characterized by the release of decondensed chromatin and granular contents to the extracellular space, forming neutrophil extracellular traps (NETs) to eliminate invading microorganisms. Thus, we confirmed the existence of a low NETosis induced in A. phagocytophilum-infected cells by immunofluorescence (IF) experiments. These results provide new insights into the complex mechanisms that govern immune response during A. phagocytophilum host interactions.
- Subjects
ANAPLASMA phagocytophilum; REACTIVE oxygen species; CELL death; EXTRACELLULAR space; OXYGEN compounds
- Publication
Vaccines, 2022, Vol 10, Issue 10, pN.PAG
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines10101756